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目的 探讨可乐定置换物 (CDS)对离体正常大鼠胰岛功能的影响。方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,RPMI164 0组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察CDS对胰岛功能的影响。放免法测定孵育液中胰岛素及胰高糖素的浓度。结果 离体胰岛胰高糖素的分泌明显受葡萄糖浓度的抑制 ,CDS显著抑制胰高糖素的分泌 ,且抑制强度明显依赖于CDS的浓度。离体胰岛胰岛素的分泌依赖于孵育液中的葡萄糖浓度 ,CDS明显刺激胰岛素的释放。Ca2 + 通道阻滞剂硝苯吡啶( 2 5 μmol/L)及K+ 通道开放剂二氮嗪 ( 10 0 μmol/L)可显著抑制胰岛素释放 ,但其作用可被CDS消除。 结论 作为咪唑啉受体的内源性配体 ,CDS可刺激离体胰岛胰岛素的释放 ,抑制胰高糖素的分泌
Objective To investigate the effects of cortisol (CDS) on islet function of isolated normal rats. Methods The islets of SD rats were isolated by collagenase and DNase digestion, cultured in RPMI1640 medium overnight, and the effect of CDS on pancreatic islet function was observed by Millipore Multiscreen system. The concentration of insulin and glucagon in the incubation solution was determined by radioimmunoassay. Results The secretion of glucagon in isolated islets was obviously inhibited by glucose concentration. CDS significantly inhibited the secretion of glucagon, and the inhibitory intensity was obviously dependent on the concentration of CDS. The secretion of insulin from the isolated islets depends on the glucose concentration in the incubation solution and CDS significantly stimulates the release of insulin. Insulin release was significantly inhibited by Ca2 + channel blocker nifedipine (25 μmol / L) and K + channel opener diazoxide (100 μmol / L), but its effect was abolished by CDS. Conclusion As an endogenous ligand for imidazolinium receptor, CDS can stimulate the release of insulin in isolated islets and inhibit glucagon secretion