论文部分内容阅读
以β-葡萄糖苷酶为切入点,分离获得具有较高β-葡萄糖苷酶活性的菌株(PT-3),在添加白芍提取物为诱导物的前提下,通过优化培养基成分,确定最佳的碳、氮源和磷源及其比例.最佳组合的产酶培养基为麦芽汁800mL,(NH4)2SO4,2g,KH2PO4,1.5g,芍药苷1g,蒸馏水200mL.在此培养基中,菌株(PT-3)对芍药苷的降解率为30%.
The β-glucosidase was used as the starting point to isolate and obtain the strain (PT-3) with high β-glucosidase activity. Under the premise of adding the extract of Paeoniae Radix Paeoniae Alba to the inducer, Good carbon, nitrogen and phosphorus sources and the ratio of the best combination of enzyme production medium for the wort 800mL, (NH4) 2SO4, 2g, KH2PO4, 1.5g, paeoniflorin 1g, 200mL of distilled water in this medium , The strain (PT-3) paeoniflorin degradation rate of 30%.