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该文作者建立了用硫氰酸胍和盐酸胍从培养的细胞和组织中提纯RNA的简便方法.与其它方法比较该法具有以下特点:①减少了RNA纯化所需时间.②避免使用高浓度还原剂如2-巯基乙醇.③避免使用超速离心机和腐蚀性溶剂.①所提RNA纯度高.
The authors have established a simple method for the purification of RNA from cultured cells and tissues using guanidine thiocyanate and guanidine hydrochloride.Compared with other methods, this method has the following features: (1) reducing the time required for RNA purification, (2) avoiding the use of high concentrations Reducing agent such as 2-mercaptoethanol.③ Avoid using ultracentrifugation and corrosive solvents.① The purity of RNA is high.