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[目的]探讨抗生素诱导肠道菌群失调在RSV感染小鼠模型上对肺部Th1/Th2的影响。[方法]RSV组小鼠鼻腔接种RSV,抗生素加RSV组小鼠鼻腔接种RSV后连续口服抗生素头孢哌酮7d,接种后d9和d16进行变应原气道激发,d19检测BALF中细胞总数和分类计数,ELISA检测细胞因子水平、肺组织病理评分以及流式细胞仪检测肺部DC亚型和成熟度。[结果](1)小鼠感染RSV后,BALF接种到Hep-2细胞可以引起典型CPE,直接免疫荧光证实肺部有RSV抗原,BALF中细胞总数升高,以淋巴细胞增加为主,肺部可见明显炎症,说明RSV感染小鼠模型建立成功;(2)与对照组比较,RSV感染组和抗生素加RSV组小鼠肺部以Th1优势应答为主;(3)与RSV组比较,抗生素加RSV组小鼠BALF中细胞总数和分类计数、IL-4、IL-10和IFN-γ水平、肺部病理评分以及肺部DC亚型和成熟度无统计学差异。[结论]RSV感染可以掩盖或者逆转抗生素诱导肠道菌群失调小鼠易被诱导的肺部Th2样反应。
[Objective] To investigate the effect of antibiotic-induced gut flora on lung Th1 / Th2 in a mouse model of RSV infection. [Method] RSV mice were intranasally inoculated with RSV, RSV mice were given RSV oral antibiotics cefoperazone 7d after intranasal inoculation and challenged with allergen airway at d9 and d16 after inoculation. Total cell number and classification of BALF in d19 Counting, ELISA detection of cytokines levels, lung histopathological score and flow cytometry lung DC subtype and maturity. [Results] (1) Inoculation of Hep-2 cells with BALF into mice caused typical CPE after mice were infected with RSV. Direct immunofluorescence confirmed the RSV antigen in the lungs and the total number of cells in the BALF increased with the increase of lymphocytes. (3) Compared with the RSV group, the antibiotics plus RSV group and the antibiotic plus RSV group had the predominant Th1 response; (3) Compared with the RSV group, There were no significant differences in the total number of cells and the number of BALF, the levels of IL-4, IL-10 and IFN-γ, the pathological score of lungs and the subtypes and maturation of lung in RSV group. [Conclusion] RSV infection can mask or reverse the Th2-like reaction in lungs easily induced by antibiotics-induced gut microbiota in mice.