论文部分内容阅读
目的:筛选火绒草中的抗炎活性部位。方法:采用系统溶剂法按照极性由低到高的顺序分离得到火绒草石油醚提取物、乙酸乙酯提取物、正丁醇萃取物、水提取物。采用酶联免疫法(ELISA)建立花生四烯酸(AA)的5-脂氧合酶(5-LOX)体外代谢途径,检测火绒草提取物抑制5-LOX活性的能力。结果:火绒草乙酸乙酯提取物对5-LOX活性有76.92%强度的抑制作用。结论:火绒草乙酸乙酯提取物具有较强的抗炎活性,可作为潜在的抗炎活性成分筛选药物。
OBJECTIVE: To screen anti-inflammatory active sites in velvetleaf. Methods: Petroleum ether extract, ethyl acetate extract, n-butanol extract and water extract of turpentine were separated by systematic solvent method according to the order of polarity from low to high. In vitro metabolic pathway of arachidonic acid (AA) 5-lipoxygenase (5-LOX) was established by enzyme-linked immunosorbent assay (ELISA). Results: Edelta ethyl acetate extract had a 76.92% inhibitory effect on 5-LOX activity. Conclusion: Edelweiss ethyl acetate extract has strong anti-inflammatory activity and can be used as a potential anti-inflammatory active ingredient screening drug.