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目的探讨GSTP1和RASSF1A基因多态性及环境因素和前列腺癌易感性之间的关系。方法采用TaqMan/MGB探针基因分型方法对103例前列腺癌患者和103例正常对照的中国汉族人群基因组DNA进行GSTP1和RASSF1A基因多态性检测,并结合环境因素应用多因素Logistic回归分析吸烟、饮酒、饮茶、猪肉和牛肉每周实际摄入量及各位点基因型与前列腺癌易感性之间的关系。结果GSTP1基因型AA、AG、GG在前列腺癌和对照人群中的比例分别为66.02%、22.33%、11.65%和67.96%、29.13%、2.91%,有统计学差异(χ2=6.35,P=0.04);RASSF1A基因型CC、CA、AA在前列腺癌和对照人群中的比例分别为88.34%,5.83%,5.83%和85.44%,12.62%,1.94%,无统计学差异(χ2=4.63,P=0.10)。多因素Logistic回归分析显示,饮茶者患前列腺癌的危险性是不饮茶者的0.40倍(95%CI,0.19～0.82),吸烟者患前列腺癌的危险性是不吸烟者的3.02倍(95%CI,1.44～6.32)。结论GSTP1和RASSF1A的基因多态性和中国汉族人群前列腺癌的发生无相关性;吸烟是前列腺癌的危险因素,饮茶是保护因素。
Objective To investigate the relationship between polymorphisms of GSTP1 and RASSF1A genes and environmental factors and their susceptibility to prostate cancer. Methods Genomic DNA of 103 Han Chinese patients with prostate cancer and 103 normal controls were genotyped by TaqMan / MGB probe to detect GSTP1 and RASSF1A gene polymorphism. Combined with environmental factors, multivariate logistic regression analysis was used to analyze the smoking, Drinking, drinking tea, weekly actual intake of pork and beef, and the relationship between site-specific genotypes and prostate cancer susceptibility. Results The proportions of GSTP1 genotypes AA, AG and GG were 66.02%, 22.33%, 11.65% and 67.96%, 29.13% and 2.91% respectively in the prostate cancer and control groups (χ2 = 6.35, P = 0.04 ); The proportions of RASSF1A genotypes CC, CA, AA in prostate cancer and control were 88.34%, 5.83%, 5.83% and 85.44%, 12.62% and 1.94% respectively, with no statistical difference (χ2 = 4.63, P = 0.10). Multivariate logistic regression analysis showed that the risk of prostate cancer in tea drinkers was 0.40 times higher than that in non-drinkers (95% CI, 0.19-0.82), and the risk of prostate cancer in smokers was 3.02 times higher than that of non-smokers 95% CI, 1.44 ~ 6.32). Conclusion There is no correlation between the polymorphisms of GSTP1 and RASSF1A and the incidence of prostate cancer in Chinese Han population. Smoking is a risk factor for prostate cancer. Tea drinking is a protective factor.