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目的探讨一氧化氮在慢性胃炎及消化性溃疡发病机制中的作用，以及ＮＯ和Ｈｐ感染的关系．方法用免疫组化法对正常对照者６例，慢性胃炎５６例及消化性溃疡患者１６例的胃粘膜标本进行检测，观察ｉＮＯＳ表达强度．并用改良Ｇｉｅｍｓａ法同步检测Ｈｐ感染状况．结果ｉＮＯＳ染色定位于胞质，在正常人胃及十二指肠粘膜细胞和腺体均有表达．慢性浅表性胃炎组呈过度表达，其平均表达强度明显高于对照组及慢性萎缩性胃炎组（Ｐ＜００１）．慢性萎缩性胃炎组平均表达水平和对照组比较差异无显著性（Ｐ＞００５）．慢性胃炎组ｉＮＯＳ表达强度与其Ｈｐ分级间呈明显正相关（Ｐ＜００５）．消化性溃疡组平均表达水平较对照组增强（Ｐ＜００５），其ｉＮＯＳ表达强度和Ｈｐ分级间相关性不明显（Ｐ＞００５）．结论ｉＮＯＳ活性增强和过度表达可能在慢性胃炎和消化性溃疡的发病机制中发挥一定作用，并可能是Ｈｐ感染导致慢性胃炎的相关发病机制之一．
Objective To investigate the role of nitric oxide in the pathogenesis of chronic gastritis and peptic ulcer and the relationship between NO and Hp infection. Methods Immunohistochemical method was used to detect the expression of iNOS in 6 cases of normal controls, 56 cases of chronic gastritis and 16 cases of peptic ulcer in gastric mucosa. Hp infection was detected simultaneously by modified Giemsa method. Results The iNOS staining was localized in the cytoplasm, which was expressed in the normal human gastric and duodenal mucosal cells and glands. The chronic superficial gastritis group was over-expressed, the average expression intensity was significantly higher than the control group and chronic atrophic gastritis group (P <001). The average expression level in chronic atrophic gastritis group was not significantly different from that in control group (P> 005). The expression of iNOS in chronic gastritis group was positively correlated with its Hp grade (P <005). The average expression level of peptic ulcer group was higher than that of control group (P <005), and the correlation between iNOS expression level and Hp grade was not significant (P> 0.05). Conclusion The enhancement and overexpression of iNOS may play a role in the pathogenesis of chronic gastritis and peptic ulcer and may be one of the pathogenesis of Hp infection leading to chronic gastritis.