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小麦的加工品质与低分子量谷蛋白亚基(LMW-GS)组成息息相关。为了尽快改良黄淮麦区小麦加工品质,应用STS分子标记与SDS-PAGE相结合的方法对小麦低分子量谷蛋白亚基Glu-A3、Glu-B3与Glu-D3位点的等位基因变异类型进行检测。结果表明:黄淮麦区南片356份小麦品种(系)中共检测到15种Glu-3位点等位变异,Glu-A3位点含Glu-A3a、Glu-A3b、Glu-A3c和Glu-A3d共4种等位变异,其分布频率分别为12.1%,13.5%,41.0%,33.4%;GluB3位点含Glu-B3a、Glu-B3b、Glu-B3d、Glu-B3f、Glu-B3g、Glu-B3h、Glu-B3i和Glu-B3j共8种等位变异,其分布频率分别为8.71%,8.99%,23.0%,5.90%,7.30%,3.65%,0.28%,42.1%;Glu-D3位点含Glu-D3a、Glu-D3b和Glu-D3c共3种等位变异,其分布频率分别为40.2%,29.8%,30.0%。等位变异组合Glu-A3c/Glu-B3j/Glu-D3a分布频率最高(10.7%)。通过优质亚基的转育,多个优质亚基的聚合,将有助于改良我国小麦的品质。
The processing quality of wheat is closely related to the composition of low molecular weight glutenin subunits (LMW-GS). In order to improve the processing quality of wheat in Huanghuai wheat area as soon as possible, the allelic variants of Glu-A3, Glu-B3 and Glu-D3 loci of wheat low molecular weight glutenin subunits were analyzed by using STS and SDS-PAGE. Test. The results showed that 15 loci of Glu-3 loci were detected in 356 wheat cultivars (lines) in the southern part of Huanghuai wheat region. Glu-A3a, Glu-A3b, Glu-A3c and Glu- A total of four kinds of allelic variation were found, which were distributed at the frequencies of 12.1%, 13.5%, 41.0% and 33.4%, respectively. GluB3, Glu-B3b, Glu- There were 8 alleles of Glu, B3h, Glu-B3i and Glu-B3j with the frequencies of 8.71%, 8.99%, 23.0%, 5.90%, 7.30%, 3.65%, 0.28% and 42.1% There were three allelic variations including Glu-D3a, Glu-D3b and Glu-D3c, with distribution frequencies of 40.2%, 29.8% and 30.0% respectively. The allele variant Glu-A3c / Glu-B3j / Glu-D3a had the highest distribution frequency (10.7%). Through the transfer of high quality subunits, the polymerization of multiple high quality subunits will help to improve the quality of wheat in our country.