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目的未经处理的菜市场污水中携带大量细菌(特别是病原细菌),查清菜市场污水中细菌多样性分布和种群结构特征,为菜市场污水治理措施提供基础数据。方法通过提取水中微生物总DNA,利用PCR扩增细菌16S rRNA基因片段,构建污水中细菌16S rRNA基因文库。结果菜市场宰杀清洗家禽和水产品产生的污水中细菌多达16个属30多种,细胞16S rRNA基因主要来自变形细菌(proteobacteria)的ε-,γ-,δ-,β-亚族、厚壁菌门(Firmiutes)和拟杆菌门(Bacteroidetes)等类群,近似弓形菌属(Arcobacter)和梭菌属(Clostridium)的细菌比例最高,分别为27.6%和20.7%。结论构建细菌16SrRNA基因文库的分子生物学方法可快速检测菜市场污水中细菌,且细胞种群呈多样性。
Objective Untreated vegetable market sewage carrying a large number of bacteria (especially pathogenic bacteria), to find out the distribution of bacterial diversity in vegetable market sewage and population structure characteristics, provide the basic data for the sewage treatment measures in the market. Methods The bacterial 16S rRNA gene library was constructed by extracting the 16S rRNA gene fragment of bacteria by PCR. As a result, more than 30 bacterial species were found in the wastewater produced by the slaughter and cleaning of poultry and aquatic products. The 16S rRNA gene of the cell mainly came from the ε-, γ-, δ-, β-subfamilies of the proteobacteria Bacteria and Bacteroidetes and other bacteria, the highest proportion of bacteria of the genus Arcobacter and Clostridium were 27.6% and 20.7%, respectively. Conclusion The molecular biology method of constructing bacterial 16S rRNA gene library can rapidly detect bacteria in the vegetable market sewage and the cell population is diversified.