论文部分内容阅读
为进一步了解我国不同地区流行的水痘—带状疱疹病毒(VZV)病毒株的基因型分布和分析代表性病毒株gE基因序列。于2010年12月至2011年6月期间,收集来自北京市、长春市、拉萨市和乌鲁木齐市4个地区水痘和带状疱疹患者的疱疹液拭子和皮肤痂片,共计18份。用单个核苷酸多态性(SNP)谱的方法确定病毒株的基因型。然后采用聚合酶链反应(PCR)方法扩增gE基因的全长片段,并进行测序分析。SNP分析显示18个病毒株的基因型共为4种,其中有7株属于clade2遗传支,1株属于clade3遗传支,4株为clade5遗传支。另有6株病毒兼有不同遗传支的特征,按照目前国际通用的分型方法不能归属于任何明确的遗传支。对不同病毒株gE基因序列分析,除了发现3个国外已有报道的1个同义突变(T660C)和2个反义突变(C119T、C1606A),还发现了3个新的反义突变(C56T、C1109T和C917A)和4个同义突变(C54T、T1075C、T816C和G279A)。首次在我国新疆自治区发现了clade5遗传支的VZV,在长春市还发现了目前尚未能分型的6株病毒。对部分病毒株gE基因序列分析,在gE的e1和c1抗原表位的编码区内中检出1个新型反义突变(C917A),需要进一步研究该突变对该病毒免疫原性和致病性的影响。
In order to further understand the genotype distribution of the chickenpox-herpes zoster virus (VZV) strains prevailing in different areas of China and to analyze the gE gene sequences of the representative virus strains. Between December 2010 and June 2011, herpes fluid swabs and skin scabs were collected from 18 patients with chickenpox and herpes zoster in four districts of Beijing, Changchun, Lhasa and Urumqi. A single nucleotide polymorphism (SNP) spectrum was used to determine the genotype of the strain. Then, the full-length fragment of gE gene was amplified by polymerase chain reaction (PCR) and sequenced. SNP analysis showed that there were 4 genotypes in 18 strains, of which 7 belonged to clade2 genetic branch, 1 belonged to clade3 genetic branch and 4 strains belonged to clade5 genetic branch. Another six strains of viruses have the characteristics of different genetic branches, according to the current international classification method can not be attributed to any clear genetic branch. In addition to 3 synonymous mutations (T660C) and 2 antisense mutations (C119T, C1606A) that were reported abroad, 3 new antisense mutations (C56T , C1109T and C917A) and 4 synonymous mutations (C54T, T1075C, T816C and G279A). For the first time in our country, Xinjiang Autonomous Region discovered the clade5 genetic branch of VZV, also found in Changchun City has not been able to type 6 viruses. A partial analysis of the gE gene sequences revealed that a novel antisense mutation (C917A) was found in the coding region of the e1 and c1 epitopes of gE, and further study was needed on the immunogenicity and pathogenicity of the mutant Impact.