论文部分内容阅读
OBJECTIVE This study discusses the effects of scutellarin(SCU),which is one of effective component of Erigeron breviscapus(Vant.)Hand-Mazz,on cGMP dependent protein kinase(PKG)in human cardiac microvascular endothelial cells(HCMECs)after hypoxia roxygenation(HR).METHODS Based on a model of HCMECs cells with HR injury,cell viability is examined by MTT assay.Protein expression of PKG is analyzed by Western blotting and its enzyme activity is investigated by ELISA technology.RESULTS The results of MTT assay- indicate that SCU(1,10μmol·L1)could protect HCMECs against HR injury.SCU(0.1,1 and 10μmol·L-1)canenhance PKG-I expression in control and HR injury cells.Furthermore,SCU(1,10μmol·L-1 significantly increase PKG activity in control cells(P<0.01),and also SCU(100μmol·L-1)appears similar on enzyme activity in HR injury cells(P <0.05).CONCLUSION SCU appears protective effects on endothelial cells against HR damage.While its mechanisms may be related to the influence of SCU on PKG activity in HCMECs.
OBJECTIVE This study discusses the effects of scutellarin (SCU), which is one of the effective components of Erigeron breviscapus (Vant.) Hand-Mazz, on cGMP dependent protein kinase (PKG) in human cardiac microvascular endothelial cells (HCMECs) after hypoxia roxygenation HR). METHODS Based on a model of HCMECs cells with HR injury, cell viability is examined by MTT assay. Protein expression of PKG is analyzed by Western blotting and its enzyme activity is investigated by ELISA technology. RESULTS The results of MTT assay- (1, 10μmol·L1) could protect HCMECs against HR injury. SCU (0.1,1 and 10μmol·L-1) canenhance PKG-I expression in control and HR injury cells. (P <0.01), and also SCU (100μmol·L-1) appeared similar on enzyme activity in HR injury cells (P <0.05) .CONCLUSIONS SCU appears protective effects on endothelial cells against HR damage .While its mechanisms may be related to the influence of SCU on PKG ac tivity in HCMECs.