论文部分内容阅读
目的：寻找观察骨骼肌小动脉形态的有效方法．方法;采用墨汁灌注、石蜡切片HE染色及血管铸型结合扫描电镜三种手段观察了大鼠比目鱼肌小动脉．并对它们的效果及优缺点进行了比较．结果：墨汁灌注法能清楚地显示整块比目鱼肌内的小动脉网;石蜡切片HE染色法能清楚地观察到血管壁断面的结构及其周围结缔组织的变化;血管铸型结合扫描电镜可观察到骨骼肌内小动脉网的立体构建．结论：上述三种方法在显示骨骼肌小动脉方面各有优缺点．应用时可根据不同的研究目的选择不同的方法．
Objective: To find an effective method to observe the morphology of skeletal muscle arterioles. Methods: The rat soleus arterioles were observed by means of ink perfusion, HE staining of paraffin sections and vascular cast combined with scanning electron microscopy. And their effects and advantages and disadvantages were compared. Results: The ink perfusion method could clearly show the arteriolar network in the whole soleus muscle. The structure of the vascular wall and the surrounding connective tissue were clearly observed by paraffin section HE staining. The results of the vascular cast and scanning electron microscopy To the skeletal muscle arteriolar network of three-dimensional construction. Conclusion: The above three methods have their own advantages and disadvantages in displaying skeletal muscle arterioles. When applied according to different research purposes choose different methods.