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目的 研究肝癌组织中ｃ－ｆｏｓ蛋白和ｃ－ｆｏｓｍ ＲＮＡ 的表达及其与乙型肝炎病毒（ＨＢＶ）的关系。 方法 采用免疫组化及原位杂交技术检测５９ 例肝细胞癌组织及５６ 例癌旁组织中ＨＢｓＡｇ、ＨＢＶ ＤＮＡ、ｃ－ｆｏｓ蛋白及ｃ－ｆｏｓｍ ＲＮＡ的表达。 结果 癌旁肝组织ＨＢｓＡｇ 阳性率为８７．５％ （４９／５６）。肝癌组织ＨＢＶ ＤＮＡ阳性率为５９．３％ （３５／５９）。肝细胞癌组织、癌旁肝组织ｃ－ｆｏｓ蛋白的阳性率分别为３９．０％ 和５３．６％ ，ｃ－ｆｏｓｍ ＲＮＡ 的阳性率分别为３３．９％ 和５０．０％ 。癌旁肝细胞不典型增生组ｃ－ｆｏｓ蛋白、ｃ－ｆｏｓｍ ＲＮＡ 的阳性率均显著高于非不典型增生组（Ｐ＜ ０．０５），非癌正常对照组均为阴性。癌组织ＨＢＶ ＤＮＡ 阳性组病例ｃ－ｆｏｓ蛋白、ｃ－ｆｏｓｍ ＲＮＡ 的阳性率显著高于癌组织ＨＢＶＤＮＡ 阴性组（Ｐ＜ ０．０５）。 结论 肝细胞癌组织中存在ｃ－ｆｏｓ癌基因的异常表达，并与ＨＢＶ ＤＮＡ有密切关系。
Objective To study the expression of c-fos protein and c-fosm RNA in hepatocellular carcinoma and its relationship with hepatitis B virus (HBV). Methods Immunohistochemistry and in situ hybridization were used to detect the expression of HBsAg, HBV DNA, c-fos protein and c-fosm RNA in 59 cases of hepatocellular carcinoma and 56 cases of adjacent tissues. Results The positive rate of HBsAg in the adjacent liver tissues was 87.5% (49/56). The positive rate of HBV DNA in HCC tissues was 59.3% (35/59). The positive rates of c-fos protein were 39.0% and 53.6% in hepatocellular carcinoma tissues and para-tumor liver tissues, respectively. The positive rates of c-fosm RNA were 33.9% and 50.0%, respectively. The positive rate of c-fos protein and c-fosm RNA in the paracancer atypical hyperplasia group were significantly higher than those in the non-atypical hyperplasia group (P<0.05). The non-cancer normal control group was negative. The positive rate of c-fos protein and c-fosm RNA in the HBV DNA positive group was significantly higher than that in the cancer tissue HBV DNA negative group (P<0.05). Conclusion The abnormal expression of c-fos oncogene in hepatocellular carcinoma is closely related to HBV DNA.