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目的探讨atelocollagen胶原支架在三维培养心肌细胞中的生物相容性,寻找三维培养心肌组织的条件和理想的支架材料。方法原代培养的大鼠心肌细胞经纯化后,将细胞悬液接种到胶原纤维支架上,动态观察第8d、16d、20d在atelocollagen胶原纤维支架上生长的细胞的变化,并进行光镜、扫描电镜和透射电镜观察。结果接种后第1d形成细胞-胶原支架搏动复合体,细胞与支架一起有节律的跳动;心肌细胞填充于支架网孔周围,并与支架融合;在透射电镜下,3个时间段均发现细胞与支架紧密相贴,融合在一起;在扫描电镜下发现,细胞在胶原纤维支架上贴附并充分伸展,相互融合成片。结论atelocollagen胶原支架的生物相容性较好,可作为三维培养细胞和组织的天然材料,适于心肌组织的立体培养。
Objective To investigate the biocompatibility of atelocollagen collagen scaffolds in three-dimensional culture of cardiomyocytes and to find the conditions and ideal scaffolds for three-dimensional culture of myocardial tissue. Methods After primary cultured rat cardiomyocytes were purified, the cell suspension was inoculated into collagen scaffolds. The changes of cells growing on atelocollagen collagen scaffolds on day 8, 16 and 20 were observed dynamically. Electron microscopy and transmission electron microscopy. Results The cell-collagen scaffold pulsatile complex was formed on day 1 after inoculation. The cells rhythms with the scaffolds. Cardiomyocytes were filled around the scaffold meshes and fused with the scaffolds. Under the transmission electron microscope, The scaffolds are closely attached to each other and fused together; under the scanning electron microscope, the cells are attached and fully extended on the collagen scaffolds and fused with each other. Conclusion atelocollagen collagen scaffold has good biocompatibility and can be used as three-dimensional culture of cells and tissues of natural materials, suitable for three-dimensional culture of myocardial tissue.