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[Objective]To determine the dynamic changes in several physiological indices of Viola yedoensis Makino under salinity,cadmium(Cd)stress and drought stresses,so as to deducing its physiological mechanism and laying foundation for further investigating the molecular mechanism and functional genes in response to abiotic stresses.[Method]Employing both hydroponic culture and potted cultivation methods,different salt concentration,Cd concentration and soil water content were set up on the plants with similar growth vigor for measuring MDA content,POD and SOD activities at various growth periods.[Results]Stressed by 0.4%Na2SO4,180μmol/L Cd and 12%of soil moisture,the physiological indices investigated showed regular change trends,indicating the graded response process of V.yedoensis to environmental factors.The activities of protective enzymes were the highest at 54 h,30 h and 3 d after salinity,Cd and drought stress respectively,when the expression of regulatory genes may peak in response to stress.[Conclusion]Whether MDA can be used as a physiological index for evaluating drought and salinity resistance of V.yedoensis still needs further experimental evidence,while the synergistic effect of protective enzymes POD and SOD can prevent cell membrane of V.yedoensis from damage caused by Cd and drought stresses.
[Objective] To determine the dynamic changes in several physiological indices of Viola yedoensis Makino under salinity, cadmium (Cd) stress and drought stresses, so as to deducing its physiological mechanism and laying foundation for further investigating the molecular mechanism and functional genes in response to [Method] Employing both hydroponic culture and potted cultivation methods, different salt concentrations, Cd concentration and soil water content were set up on the plants with similar growth vigor for measuring MDA content, POD and SOD activities at various growth periods. [ Results] Stressed by 0.4% Na2SO4, 180 μmol / L Cd and 12% of soil moisture, the physiological indices showing saw regular change trends, indicating the graded response process of V.yedoensis to environmental factors. The activities of protective enzymes were the highest at 54 h, 30 h and 3 d after salinity, Cd and drought stress respectively, when the expression of regulatory genes may peak in response [Conclusion] Whether MDA can be used as a physiological index for evaluating drought and salinity resistance of V.yedoensis still needs further experimental evidence, while the synergistic effect of protective enzymes POD and SOD can prevent cell membrane of V.yedoensis from damage caused by Cd and drought stresses.