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目的观察实验动物对叶酸偶联二氧化硅包覆的金纳米棒(GNRs@SiO2-FA)的摄取状况,检测GNRs@SiO2-FA对肝癌细胞的靶向性。方法超声引导下穿刺接种法建立兔VX2肝癌模型27只,超声检查观察肿瘤生长情况。2周后将实验动物随机分成三组,即空白对照组(注入生理盐水)、门静脉注射GNRs@SiO2-FA组、瘤内注射GNRs@SiO2-FA组,术后24、48、72h每组各处死3只实验兔,取其肿瘤组织和各主要脏器观察GNRs@SiO2-FA摄取情况。结果成功建立VX2兔肝癌模型,实验动物摄入GNRs@SiO2-FA后,在24h内GNRs@SiO2-FA即可以特异度的与肿瘤细胞结合进入肿瘤细胞,并聚集在肿瘤细胞质内,瘤内注射组与门静脉注射组无明显差异。结论 GNRs@SiO2-FA可以在实验动物体内高度靶向性作用于肝癌细胞。
Objective To investigate the uptake of GNRs @ SiO2-FA by the lab animal and to investigate the targeting of GNRs @ SiO2-FA to hepatoma cells. Methods Twenty-seven VX2 hepatocellular carcinoma models were established by puncture inoculation guided by ultrasound. Tumor growth was observed by ultrasound. Two weeks later, the experimental animals were randomly divided into three groups: blank control group (saline injection), GNRs @ SiO2-FA group and GNRs @ SiO2-FA group Three experimental rabbits were sacrificed and their tumor tissues and major organs were observed for GNRs @ SiO2-FA uptake. Results VX2 rabbit liver cancer model was successfully established. GNRs @ SiO2-FA could specifically bind with tumor cells into tumor cells within 24 hours after ingestion of GNRs @ SiO2-FA by experimental animals, and accumulated in tumor cytoplasm and intratumoral injection Group and portal vein injection group no significant difference. Conclusions GNRs @ SiO2-FA can be highly targeted to hepatoma cells in experimental animals.