论文部分内容阅读
NEK6基因编码一种丝/苏氨酸蛋白激酶,具有调控植物细胞周期蛋白基因表达和乙烯信号转导的功能,与植物抵抗逆境胁迫相关。将拟南芥At NEK6基因导入烟草,利用甘露醇和Na Cl模拟干旱和盐胁迫环境对T2代转基因烟草进行研究。结果表明,在无胁迫处理条件下,转基因烟草根长、侧根数和鲜重显著高于野生型;在干旱和盐胁迫条件下,转基因烟草根长、侧根数和鲜重均极显著高于野生型,表现出较强的耐旱耐盐性;胁迫处理的转基因烟草叶片叶绿素荧光参数值(Fv/Fm)、SOD和CAT活性以及Pro含量极显著高于野生型,MDA含量极显著低于野生型,进一步证明了At NEK6基因能够促进烟草的生长和提高转基因烟草对于干旱与盐碱的抵抗能力。
The NEK6 gene encodes a silk / threonine protein kinase that regulates plant cell cycle protein gene expression and ethylene signaling and is associated with plant resistance to stress. The Arabidopsis thaliana At NEK6 gene was introduced into tobacco and the T2 generation transgenic tobacco was studied by simulating the drought and salt stress environment with mannitol and NaCl. The results showed that the root length, lateral root number and fresh weight of transgenic tobacco were significantly higher than those of wild type under the condition of no stress treatment. Under drought and salt stress conditions, the root length, lateral root number and fresh weight of transgenic tobacco were significantly higher than wild (Fv / Fm), the activity of SOD and CAT and the content of Pro in the transgenic tobacco leaves were significantly higher than those in the wild type, and the content of MDA in the transgenic tobacco leaves was significantly lower than that in the wild It further proves that At NEK6 gene can promote the growth of tobacco and improve the resistance of transgenic tobacco to drought and salinity.