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目的探讨KAI1基因在喉鳞状细胞癌中的表达及其临床意义。方法取40例喉鳞癌及9例正常对照喉组织,应用半定量的RT PCR方法,检测KAI1基因mRNA的表达。结果正常喉黏膜KAI1基因mRNA的中、低表达率和阴性率均为33.3%(3/9);无淋巴结转移组喉癌高、中、低表达率和阴性率分别为40.0%(10/25)、28.0%(7/25)、20.0%(5/25)和12.0%(3/25);淋巴结转移组喉癌中、低表达率和阴性率分别为20.0%(3/15)、26.7%(4/15)和53.3%(8/15),无淋巴结转移组喉癌与正常喉组织比较,KAI1mRNA表达升高差异有统计学意义(P<0.05),有淋巴结转移与无淋巴结转移组喉癌比较,KAI1基因mRNA表达下降差异有统计学意义(P<0.05)。病理高分化喉癌KAI1基因mRNA高、中、低表达率分别为50.0%(5/10)、30.0%(3/10)和20.0%(2/10);病理低分化喉癌其高、中、低表达率和阴性率分别为8.3%(1/12)、16.7%(2/12)、16.7%(2/12)和58.3%(7/12);高分化与低分化比较,KAI1基因mRNA表达差异有统计学意义(P<0.05)。结论KAI1基因mRNA表达下降可能与喉鳞状细胞癌的病理低分化和淋巴结转移有关。
Objective To investigate the expression of KAI1 in laryngeal squamous cell carcinoma and its clinical significance. Methods 40 cases of laryngeal squamous cell carcinoma and 9 cases of normal control laryngeal tissue were obtained. Semi-quantitative RT-PCR was used to detect the expression of KAI1 gene mRNA. Results The median, low and negative rates of KAI1 mRNA in normal laryngeal mucosa were 33.3% (3/9) respectively. The high, moderate, low and low rates of laryngeal carcinoma with no lymph node metastasis were 40.0% (10/25) ), 28.0% (7/25), 20.0% (5/25) and 12.0% (3/25) respectively. The low and negative rates of lymph node metastasis were 20.0% (3/15), 26.7 (4/15) and 53.3% (8/15) respectively. There was significant difference in the expression of KAI1mRNA between laryngeal carcinoma without lymph node metastasis and normal laryngeal tissues (P <0.05), with lymph node metastasis and without lymph node metastasis Compared with laryngeal carcinoma, the expression of KAI1 mRNA decreased significantly (P <0.05). The high, middle and low expression rates of KAI1 gene in well-differentiated laryngeal carcinoma were 50.0% (5/10), 30.0% (3/10) and 20.0% (2/10), respectively. , Low expression rate and negative rate were 8.3% (1/12), 16.7% (2/12), 16.7% (2/12) and 58.3% (7/12), respectively. The difference of mRNA expression was statistically significant (P <0.05). Conclusion The decreased expression of KAI1 mRNA may be related to the poorly differentiated and lymph node metastasis of laryngeal squamous cell carcinoma.