The transient transfection of survivin-targeted siRNA to LOVO cells and its influence on the biological features were studied.Two pairs of 19 base Pairs (bp) siRNAspecific targeted survivin gene were designed and synthesized by in vitro transcription(Survivin-1,Survivin-2).After transient transfection of the two survivin-targeted siRNAs to Lovo cells by LipofectamineTM 2000,the expression of survivin mRNA was detected by reverse transcriptionpolymerase chain reaction(RT-PCR).Apoptosis was detected by flow cytometry and cell proliferation was evaluated by MTT assay.We found that the expression levels of survivin mRNA of the two RNAi groups(Survivin-1 group and Survivin-2 group)respectively decreased by 70%and 39.1% compared with the control Lovo’s.Seventy-two hours after transfection.apoptosis rates of the two RNAi groups were 21.51%and 26.28%,both of which were higher than control Lovo’s (9.03%).The results at 72 h after transfection were mat the optical density (OD) at 490 nm of the two RNAi groups was 0.581±0.070 and 0.681±0.104.both of which were much lower than the control Lovo’s (2.060±0.272).Based on the results,we can draw a conelusion that the two survivin-targeted siRNAS successfully suppressed the expression of survivin mRNA,inhibited cell growth and induce cell apoptosis.It provides a powerful evidence for colorectal carcinoma gene therapy.