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目的 :探讨奥美拉唑对大鼠胃上皮凋亡的影响。方法 :将 1 4只Wistar大鼠随机分为 2组 :实验组予以奥美拉唑 (40mg/kg)灌胃 1次 /d ,共 7d ;对照组以生理盐水代替。 7d后处死动物 ,分别取胃底、胃体、胃窦部黏膜标本 ,用TUNEL法检测胃上皮细胞凋亡情况。结果 :对照组、实验组胃底、胃窦、胃体部凋亡指数分别为 :4.98± 1 .64、8.1 2± 2 .61 ;4.69± 1 .58、7.2 9± 2 .34 ;3 .82± 1 .52、6 .68±2 .65 ,实验组与对照组相比 ,有显著性差异 (P <0 .0 5) ,两组组内比较无显著性差异 (P >0 .0 5)。结论 :奥美拉唑在体内能诱导大鼠胃上皮细胞凋亡 ,凋亡增加可能是过度增殖和突变的刺激因素 ,或是对增殖的反馈平衡调节
Objective: To investigate the effects of omeprazole on gastric epithelial apoptosis in rats. Methods: One hundred and four Wistar rats were randomly divided into two groups: the experimental group was given omeprazole (40mg / kg) once a day for 7 days, and the control group was replaced by normal saline. The animals were sacrificed on day 7, mucosa samples of gastric fundus and gastric antrum were collected, and the apoptosis of gastric epithelial cells was detected by TUNEL method. Results: The apoptotic indexes of gastric mucosa, gastric antrum and gastric body in control group and experimental group were 4.98 ± 1 .64, 8.12 ± 2.61, 4.69 ± 1.58 and 7.29 ± 2.34, respectively. 82 ± 1.52,6.68 ± 2.65, the experimental group compared with the control group, there was significant difference (P <0.05), there was no significant difference between the two groups (P> 0 .0 5). CONCLUSION: Omeprazole can induce apoptosis of gastric epithelial cells in rats in vivo. Increased apoptosis may be the stimulus for hyperproliferation and mutation, or the feedback balance regulation of proliferation