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目的:研究地鳖纤溶活性蛋白(EFP)对H22和S180荷瘤小鼠肿瘤组织微血管密度(MVD)及血液中血管内皮细胞生长因子(VEGF)的影响,以判定EFP是否具有抗肿瘤新生血管生成作用。方法:建立H22荷瘤小鼠动物模型,采用免疫组化方法标记CD34,检测了EFP对小鼠H22和S180肿瘤组织MVD的作用情况,采用双抗体夹心ELISA法检测H22和S180荷瘤小鼠血清中VEGF含量。结果:与对照组相比,EFP可显著降低MVD及VEGF的表达。结论:EFP有抑制肿瘤新生血管形成的作用。
Objective: To study the effect of EFP on tumor microvessel density (MVD) and blood vascular endothelial growth factor (VEGF) in H22 and S180 tumor-bearing mice to determine whether EFP has antitumor neovascularization effect. Methods: The animal model of H22 tumor-bearing mice was established. The expression of CD34 was detected by immunohistochemistry. The effect of EFP on the MVD in mice H22 and S180 was detected. The serum levels of H22 and S180 tumor-bearing mice were detected by double antibody sandwich ELISA In the VEGF content. Results: Compared with the control group, EFP significantly reduced the expression of MVD and VEGF. Conclusion: EFP can inhibit tumor neovascularization.