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以色季拉山的高寒植物垫状雪灵芝为材料,选取分布在海拔4500~4650m范围内的垫状雪灵芝3个居群为研究对象,采用聚丙烯酰胺琼脂电泳技术,对垫状雪灵芝3个居群的48个个体功能叶片的酯酶同工酶谱带进行测定并编码,依据酶谱带测定结果进行聚类分析;同时结合酯酶同工酶的3个遗传指标进行相关分析。结果表明:来自同一居群的个体,同工酶谱带相似性较大,居群内的遗传杂合度较小(HeA=0.735,HeB=0.765,HeC=0.726),这也说明居群内个体存在一定程度的遗传分化;酯酶在不同居群间的相似性系数的平均值相对较小,遗传距离相对较大(IAB=0.392,IAC=0.542,IBC=0.610;DAB=0.937,DAC=0.613,DBC=0.495)。研究数据显示垫状雪灵芝酯酶同工酶在不同的居群内与居群间存在不同程度的遗传分化,缺少有效的基因交流。
In this study, three populations of Cochinchina pluvialis distributed in the range of 4500 ~ 4650 m above sea level were selected as research objects. The polyacrylamide agar electrophoresis was used to study the effects of Ganoderma lucidum Esterase isoenzyme bands of 48 individual functional leaves of 3 populations were determined and encoded, and cluster analysis was performed based on the results of zymogram determination. Meanwhile, the correlation analysis was carried out with 3 genetic indexes of esterase isozymes. The results showed that the individuals from the same population had similar isozyme bands and smaller genetic heterozygosity (HeA = 0.735, HeB = 0.765, HeC = 0.726), which also indicated that individuals within the population There was a certain degree of genetic differentiation. The average of the similarity coefficient of esterase in different populations was relatively small and the genetic distance was relatively large (IAB = 0.392, IAC = 0.542, IBC = 0.610; DAB = 0.937, DAC = 0.613 , DBC = 0.495). The data show that C. lucorum esterase isozymes in different populations and populations have different degrees of genetic differentiation, the lack of effective gene exchange.