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目的探讨能被临床普遍应用的反映胃炎、胃上皮内瘤和胃癌临床病理特征的分子标志物。方法应用免疫组化法检测34例慢性胃粘膜炎、26例低级别胃上皮内瘤、21例高级别胃上皮内瘤及30例胃癌标本中P53、CyclinD1及PCNA的基因表达。结果P53、CyclinD1及PCNA基因表达在慢性胃粘膜炎组、低级别胃上皮内瘤组、高级别胃上皮内瘤组及胃癌组间比较差异有统计学意义(P<0.01)。P53在低级别胃上皮内瘤组与高级别胃上皮内瘤组比较差异无统计学意义(P>0.05)。CyclinD1在慢性胃粘膜炎组与低级别胃上皮内瘤组,高级别胃上皮内瘤组与胃癌组比较差异均无统计学意义(P>0.05)。结论P53、CyclinD1及PCNA在胃炎、胃上皮内瘤及胃癌的发生、发展中起重要作用,是反映胃肿瘤生物学行为和预后的重要标记物。
Objective To explore molecular markers that can be widely used in clinic to reflect the clinicopathological features of gastritis, intraepithelial neoplasia and gastric cancer. Methods The expressions of P53, CyclinD1 and PCNA in 34 cases of chronic gastric mucositis, 26 cases of low grade intraepithelial neoplasia, 21 cases of high grade intraepithelial neoplasia and 30 cases of gastric cancer were detected by immunohistochemistry. Results The expressions of P53, CyclinD1 and PCNA were significantly different between chronic gastric mucositis group, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia and gastric cancer group (P <0.01). There was no significant difference in P53 between low-grade intraepithelial neoplasia and high-grade intraepithelial neoplasia (P> 0.05). CyclinD1 showed no significant difference between chronic gastric mucositis group and low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia and gastric cancer group (P> 0.05). Conclusions P53, CyclinD1 and PCNA play an important role in the occurrence and development of gastritis, intraepithelial neoplasia and gastric cancer and are important markers reflecting the biological behavior and prognosis of gastric tumors.