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作者以兔腹腔巨噬细胞条件培养基(Mφ-CM)作为生长因子的来源,研究巨噬细胞源性生长因子(MDGF)对动脉内膜内平滑肌细胞(SMC)增生的影响。他们用流式细胞计及显微分光光度计测定细胞群体内单个SMC的DNA含量,结合相差显微镜观察,研究其对培养的平滑肌细胞细胞周期进程的影响。结果表明,5％PPS组培养72h后,G_0/G_1期细胞数增加,S期细胞数减少,而G_2M期细胞数显著增多,直方
The author used rabbit peritoneal macrophage conditioned medium (Mφ-CM) as a growth factor source to study the effect of macrophage-derived growth factor (MDGF) on proliferation of intimal smooth muscle cells (SMCs). Using flow cytometry and micro-spectrophotometer, they measured the DNA content of single SMC in the cell population, and observed their effect on the cell cycle progression of cultured smooth muscle cells by phase contrast microscopy. The results showed that after cultured in 5% PPS for 72h, the number of G 0 / G 1 phase cells increased, the number of S phase cells decreased, while the number of G 2 M phase cells increased significantly,