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用原位杂交法和ABG法对慢性活动性肝炎患者(以下称CAH)血清HBsAg阴性34例和阳性30例对比进行了肝内HBV DNA、HBcAg和HBsAg的检测。上述检出率在34例HBsAg阴性CAH者中分别为23.5％,23.5％和32.4％,30例HBsAg阳性者中分别为63.3％、56.7％和83.3％。结果表明,在我国,血清HBsAg阴性、HBV相关抗体阳性或阴性的CAH中,仍有少部分人肝内存在HBV DNA和HBV抗原表达,并与肝病发病机理有关。血清HBsAg阳性的CAH者中,无论血清HBeAg阳性,或抗HBe阳性、或HBeAg阴性,均有较高比例的肝内HBV DNA和HBV抗原检出。肝内HBcAg与肝内HBV DNA关系密切,可作为反映HBV活跃复制指标;肝内HBsAg则不然。
In situ hybridization and ABG method were used to detect intrahepatic HBV DNA, HBcAg and HBsAg in 34 patients with chronic active hepatitis (hereinafter referred to as CAH) and 30 patients with positive HBsAg negative. The detection rate was 23.5%, 23.5% and 32.4% in 34 cases of HBsAg-negative CAH and 63.3%, 56.7% and 83.3% in 30 cases of HBsAg-positive respectively. The results showed that in our country, there are still a few of human liver HBV DNA and HBV antigens in serum of HBsAg-negative and HBV-related antibody-positive or negative CAH, which is related to the pathogenesis of liver disease. Serum HBsAg-positive CAH, regardless of serum HBeAg-positive, or anti-HBe-positive, or HBeAg-negative, a higher proportion of intrahepatic HBV DNA and HBV antigens were detected. Intrahepatic HBcAg and HBV DNA in the liver are closely related, can be used as an indicator of active replication of HBV; intrahepatic HBsAg is not.