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目的检测MiR-150在鼻咽癌侧群(SP)细胞中的表达,并探讨其是否通过调控靶基因Nanog促进鼻咽癌侧群细胞的增殖与侵袭。方法采用SYBR Green实时定量荧光聚合酶链式反应(qRT-PCR)法检测MiR-150及Nanog在鼻咽癌侧群细胞和非侧群(NSP)细胞中的表达情况;并通过瞬时转染miR-150 inhibitor至SP细胞、miR-150 mimic至NSP细胞中,w estern blotting检测Nanog的表达情况;进一步通过CCK-8实验、Transw all实验检测鼻咽癌侧群细胞增殖、侵袭能力的变化,数据处理采用两独立样本t检验。结果 qRT-PCR检测结果表明,M iR-150在SP细胞(0.99±0.05)较NSP细胞(0.59±0.02)中表达水平升高(t=8.06,P<0.000 1),Nanog在鼻咽癌SP细胞(0.99±0.47)较NSP细胞(0.49±0.05)表达升高(t=7.5,P<0.000 1);SP细胞转染M iR-150 inhibitor(0.46±0.03)较对照组(1.01±0.07)Nanog表达下调(t=6.85,P=0.000 5)、CCK-8实验示增殖受抑制、Transw ell侵袭实验示侵袭能力减弱(114.40±5.14 vs 57.30±4.29,t=8.5,P<0.000 1);NSP细胞转染MiR-150 mimic(1.01±0.06)组较对照组(0.48±0.04)Nanog表达上调(t=6.16,P=0.000 8),增殖及侵袭能力增强。结论鼻咽癌SP细胞中M iR-150与Nanog呈高表达,M iR-150可通过调控靶基因Nanog促进鼻咽癌侧群细胞的增殖与侵袭。
Objective To detect the expression of MiR-150 in nasopharyngeal squamous cell (SP) cells and to explore whether miR-150 promotes the proliferation and invasion of nasopharyngeal carcinoma colorectal cancer cells by regulating the target gene Nanog. Methods SYR Green quantitative real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of MiR-150 and Nanog in colorectal and non-colony-forming cells (NSP) -150 inhibitor to SP cells, miR-150 mimic to NSP cells, and Western blotting was used to detect the expression of Nanog. Further, CCK-8 and Transw all assays were used to detect the proliferation and invasion ability of NPC cells. Data The treatment uses two independent sample t tests. Results The results of qRT-PCR showed that the expression level of M iR-150 was significantly higher in SP cells (0.99 ± 0.05) than in NSP cells (0.59 ± 0.02) (t = 8.06, P <0.0001) (0.99 ± 0.47) was higher than that of NSP cells (0.49 ± 0.05) (t = 7.5, P <0.0001) The expression of Nanog was down-regulated (t = 6.85, P = 0.0005). The CCK-8 assay showed that the proliferation was inhibited. The invasion ability was weakened by Transwell assay (114.40 ± 5.14 vs 57.30 ± 4.29, t = 8.5, P <0.0001) Compared with the control group (0.48 ± 0.04), the expression of Nanog in NSP cells transfected with MiR-150 mimic (1.01 ± 0.06) was up-regulated (t = 6.16, P = 0.0008) and the proliferative and invasive ability was enhanced. Conclusion M iR-150 and Nanog are highly expressed in nasopharyngeal carcinoma SP cells. M iR-150 can promote the proliferation and invasion of NPC cells by regulating the target gene Nanog.