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目的探讨白细胞介素(IL)-17A基因启动子区域rs2275913和rs8193036基因多态性与中国西北地区汉族人群冠状动脉粥样硬化性心脏病(冠心病)的发病易感性。方法选取2012年10月至2015年8月收治的113例冠心病患者为冠心病组,36例健康体检者为对照组。以酶联免疫吸附(Elisa)法检测外周血血浆IL-17A浓度。用限制性片段长度多态性聚合酶链反应(PCR-RFLP)检测IL-17A基因启动子区域rs2275913和rs8193036的基因多态性。结果与对照组相比,冠心病组外周血IL-17A浓度显著升高[(39.97±9.09)pg/ml vs(27.88±6.68)pg/ml,P<0.01];IL-17A启动子位点rs2275913基因型AA、AG、GG分布和等位基因频率无明显差异(P均>0.05);启动子位点rs8193036基因型CC、CT、TT分布和等位基因频率差异有统计学意义(P均<0.05),且冠心病组C等位基因频率显著高于对照组(P<0.05)。结论 IL-17启动子位点rs8193036基因多态性可能与冠状动脉粥样硬化的发生有关,且rs8193036位点C等位基因携带者更容易患冠心病。
Objective To investigate the susceptibility of rs2275913 and rs8193036 polymorphisms in IL-17A gene promoter region to coronary atherosclerotic heart disease (CHD) in Han nationality in Northwest China. Methods One hundred and thirteen patients with coronary heart disease admitted from October 2012 to August 2015 were selected as coronary heart disease group and 36 healthy subjects as control group. Peripheral blood plasma IL-17A concentration was detected by Elisa method. The gene polymorphism of rs2275913 and rs8193036 in promoter region of IL-17A gene was detected by restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP). Results Compared with the control group, the concentration of IL-17A in the peripheral blood of CHD patients was significantly increased [(39.97 ± 9.09) pg / ml vs (27.88 ± 6.68) pg / ml, P <0.01] rs2275913 genotype AA, AG, GG distribution and allele frequencies were not significantly different (P all> 0.05); promoter rs8193036 genotypes CC, CT, TT distribution and allele frequency difference was statistically significant (P <0.05). The frequency of C allele in CHD group was significantly higher than that in control group (P <0.05). Conclusion rs8193036 gene polymorphism at IL-17 promoter may be related to the development of coronary atherosclerosis, and carriers of rs8193036 C allele are more susceptible to coronary heart disease.