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造血干细胞最本质的特性之一是其具有很强的自我更新能力。本文就当前各国学者所采用的研究自我更新力的方法作一概述。其中包括:(1)单个脾结节连续移植法;(2)骨髓连续移植法;(3)体内重建造血组织的能力;(4)在体外液体培养中细胞繁殖与长期活存;(5)体外集落转移的克隆分析技术。
One of the most essential properties of hematopoietic stem cells is their ability to self-renew. This article gives an overview of the methods used by scholars in various countries to study self-renewal. Including: (1) a single splenic nodule continuous transplantation; (2) bone marrow transplantation; (3) in vivo reconstruction of hematopoietic tissue capacity; (4) in vitro liquid culture cell proliferation and long-term survival; Clone Analysis Technique of Colony Transfer in.