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目的:检测金黄色葡萄球菌染色体上编码中毒休克综合征毒素-I(TSST-I)的tst基因,研究tst基因携带情况以及临床感染特点。方法:应用PCR方法对临床分离的金黄色葡萄球菌mecA基因和tst基因进行体外扩增;多重PCR对tst基因阳性耐甲氧西林金黄色葡萄球菌(MRSA)进行SCCmec基因分型。结果:460株金黄色葡萄球菌检测出mecA基因阳性180株,阳性率为39.1%(180/460);tst基因阳性菌株105株,阳性率为22.8%(105/460)。105株tst基因阳性菌株中耐甲氧西林金黄色葡萄球菌(MRSA)53株,甲氧西林敏感金黄色葡萄球菌(MSSA)52株,分别占29.4%和18.6%,MRSA较MSSA tst基因检出率高,差异有统计学意义(P<0.05)。tst基因阳性MRSASCCmec基因分型以SCCmecII和SCCmecIII为主,分别占30.2%(16/53)、60.4%(32/53),另有5株未分型。结论:tst基因阳性菌株在临床分离的MRSA中占有较高比例,SCCmec基因分型以SCCmecII和SCCmecIII为主,临床医生应予以足够重视。
Objective: To detect the tst gene encoding toxic shock syndrome toxin-I (TSST-I) on the chromosome of Staphylococcus aureus to study the characteristics of tst gene carrier and clinical infection. Methods: The clinical isolates of mecA and tst from clinical isolates of Staphylococcus aureus were amplified by PCR. The SCCmec genotypes of methicillin-resistant Staphylococcus aureus (MRSA) were analyzed by multiplex PCR. Results: 460 strains of Staphylococcus aureus were detected positive mecA gene 180, the positive rate was 39.1% (180/460); tst gene positive strains 105 strains, the positive rate was 22.8% (105/460). Among 105 strains of tst gene positive, 53 strains were methicillin-resistant Staphylococcus aureus (MRSA) and 52 strains were methicillin-sensitive Staphylococcus aureus (MSSA), accounting for 29.4% and 18.6% respectively. MRSA was detected more than MSSA tst gene High rate, the difference was statistically significant (P <0.05). The genotypes of tst gene positive MRSASCCmec were mainly SCCmecII and SCCmecIII, accounting for 30.2% (16/53) and 60.4% (32/53) respectively, and another 5 were not typed. Conclusion: The positive strains of tst gene occupy a high proportion of clinically isolated MRSA. The SCCmec genotypes are mainly SCCmecII and SCCmecIII, and clinicians should pay enough attention to it.