为了检测 Waller变性时损伤部远侧段的溃变过程是同时发生还是自切断处逐渐向远侧离心式传递 ,本实验切断大鼠坐骨神经 ,将整个远侧段分为连续的 A、B、C三段 ,A段为最靠近切断处的部位。用 c-fos免疫组化方法和 EA50 组化方法 ,比较了三段之间的形态变化和阳性细胞数。结果显示 ,神经切断后 2 d,A、B、C三段间 c-fos阳性细胞数量有显著差异。至第 5天 ,这种差别消失 ,各段间阳性细胞数量表达无显著差异。实验结果说明 ,周围神经切断后 ,信息传递是由近至远地发生 ,也说明 Waller变性是由近至远地发生。至于神经切断后 ,引起 c-fos表达的机制还有待进一步研究 In order to detect whether the degenerative process of the distal segment of the lesion during the Waller degeneration is occurring simultaneously or gradually from the severing point to the distal direction, the sciatic nerve of the rat is severed. The entire distal segment is divided into consecutive A, B, C Three sections, A section is closest to cut off the site. The c-fos immunohistochemical method and EA50 histochemical method were used to compare morphological changes between the three sections and the number of positive cells. The results showed that there were significant differences in the number of c-fos positive cells between A, B and C two days after nerve transection. By the fifth day, the difference disappeared, and there was no significant difference in the number of positive cells among the sections. The experimental results show that the peripheral nerve cut off, the transmission of information from near to far, also shows Waller degeneration occurs from near to far. As for the mechanism of c-fos expression after nerve transection remains to be further studied