在油菜小孢子培养中,通过对供体植株两种生长状况和在NLN液体培养基中三种小孢子密度的研究表明,来自生长室的供体植株,每天摘除将开放的花朵,使其停留在蕾期阶段,油菜花蕾中处于单核期至双核期的小孢子同步化程度高,单核期小孢子核质比大,增长速度快,小孢子成胚的百分率高。平均每毫升NLN液体培养基中接种1个花蕾的小孢子的处理,成胚率最高,而且胚的分化较快。 In canola microspore culture, studies of two growth conditions of donor plants and three microspore densities in NLN broth showed that donor plants from the growth chamber were harvested each day by removing the open flowers During the bud stage, the microspores in the rape buds had a high degree of synchronization between the single nucleus and the dinucleus, and the percentage of microspore nuclei and cytoplasm in the mononuclear phase was higher than that of the microspores. The average number of microspores inoculated with 1 flower bud per ml of NLN broth was the highest, and embryo differentiation was faster.