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目的对比分析不同梅毒检测方法的应用以及效果评价。方法随机抽取110例梅毒患者作为研究对象,将其作为评价组;将同时期收治的110例非梅毒患者设为对照组;采取化学发光法、梅毒苯胺红不加热血清反应素试验(TRUST)、梅毒螺旋体明胶凝集实验(TPPA)和金标测试法对两组患者进行检验,并比较两组患者检验后的阳性率、特异性、灵敏度和对照组中出现的假阳性情况。结果评价组中化学发光法、TRUST、TPPA和金标测试法的阳性检出例数分别为98例、94例、104例和105例,阳性率分别为89.09%、85.45%、94.55%和95.45%;TPPA的假阳性率与化学发光法、TRUST和金标测试法的假阳性率比较差异具有统计学意义(P<0.05);TRUST的特异性和灵敏度均低于化学发光法、TPPA和金标测试法,组间差异具有统计学意义(P<0.05)。结论化学发光法、TRUST、TPPA和金标测试法四种检测方式相比之下,TPPA法比较适合用作检验梅毒螺旋抗体,因其阳性检出率、特异性和灵敏度较高,假阳性率不明显,综合效果甚佳。
Objective To compare and analyze the application of different syphilis detection methods and their effects evaluation. Methods A total of 110 cases of syphilis were selected as the evaluation group and 110 cases of non-syphilis patients were enrolled as control group. Chemiluminescence method, syphilis aniline red serum-free reaction (TRUST) Treponema pallidum gelatin agglutination test (TPPA) and gold standard test method for testing two groups of patients, and compared the positive rate of the two groups of patients after testing, specificity, sensitivity and false positives in the control group. Results The positive detection rates of chemiluminescence, TRUST, TPPA and gold standard in the evaluation group were 98 cases, 94 cases, 104 cases and 105 cases respectively, the positive rates were 89.09%, 85.45%, 94.55% and 95.45 %. The false positive rate of TPPA was significantly lower than that of chemiluminescence, TRUST and gold standard (P <0.05). The specificity and sensitivity of TRUST were lower than that of chemiluminescence, TPPA and gold Standard test method, the difference between groups was statistically significant (P <0.05). Conclusion Compared with the four detection methods of chemiluminescence, TRUST, TPPA and gold standard test, the TPPA method is more suitable for the detection of syphilis spiral antibodies, because of its positive detection rate, specificity and sensitivity, the false positive rate Not obvious, the combined effect is very good.