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目的:建立清热解毒浸膏中白头翁皂苷B4的含量测定方法。方法:采用RP-HPLC法,色谱柱:Kromasil C18(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸(28∶72)为流动相,流速:1.0 mL.min-1,检测波长:205 nm,柱温:30℃。结果:白头翁皂苷B4在0.294~1.47μg范围内呈良好线性关系,相关系数为0.9999,平均回收率为99.6%,RSD为1.37%。结论:该方法简便、准确、重复性好,可作为清热解毒浸膏质量控制指标之一。
Objective: To establish a method for the determination of Pulsatilla saponin B4 in Qingrejiedu extract. Methods: The RP-HPLC method was used. The chromatographic column was Kromasil C18 (250 mm×4.6 mm, 5 μm) with acetonitrile-0.1% phosphoric acid (28:72) as the mobile phase. The flow rate was 1.0 mL.min-1. The detection wavelength was: 205 nm, column temperature: 30°C. Results: Pulsatilla saponin B4 showed a good linear relationship in the range of 0.294~1.47μg. The correlation coefficient was 0.9999, the average recovery was 99.6%, RSD was 1.37%. Conclusion: This method is simple, accurate, and reproducible. It can be used as one of the quality control indexes of heat-clearing and detoxifying extracts.