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目的体外、原位实验观察HRP/IAA酶前药系统对鼻咽癌C666-1细胞的杀伤效应,以探讨其对鼻咽癌的治疗作用。方法细胞转染获得稳定表达HRP蛋白的C666-1细胞。MTT法检测不同浓度的IAA对HRP表达阳性的C666-1细胞的杀伤效应。原位基因治疗:取5×106对数生长期的C666-1细胞接种裸鼠右腋下,建立鼻咽癌裸鼠移植瘤模型,接种细胞10d后将裸鼠随机分成3组,观察HRP/IAA酶前药系统对移植瘤生长的抑制作用。结果体外实验中,同一浓度IAA干预后C666-1/HRP(+)组细胞的相对存活率显著低于C666-1/HRP(-)组(P<0.05),且随着IAA浓度的升高C666-1/HRP(+)组细胞的相对存活率逐渐降低,分别为(15.58±1.12)%、(10.11土1.06)%、(5.21±1.04)%;原位基因治疗实验结果表明IAA组鼻咽癌移植瘤生长受到抑制,体积明显小于空病毒组和生理盐水组(P<0.05),且肿瘤细胞凋亡显著增多(P<0.05)。结论 HRP/IAA酶前药系统对鼻咽癌C666-1细胞具有杀伤作用,具有抗鼻咽癌的作用。
Objective To investigate the killing effect of HRP / IAA prodrug system on human nasopharyngeal carcinoma C666-1 cells in vitro and in vivo to explore its therapeutic effect on nasopharyngeal carcinoma. Methods C666-1 cells stably expressing HRP protein were obtained by cell transfection. The cytotoxicity of different concentrations of IAA on HRP-positive C666-1 cells was detected by MTT assay. In situ gene therapy: C666-1 cells in logarithmic growth phase were inoculated into the right armpit of nude mice to establish a xenograft model of nasopharyngeal carcinoma in nude mice. After being seeded for 10 days, nude mice were randomly divided into 3 groups. HRP / Inhibitory effect of IAA enzyme prodrug system on the growth of xenografts. Results In vitro, the relative survival rate of C666-1 / HRP (+) group was significantly lower than that of C666-1 / HRP (-) group after IAA intervention (P <0.05), and with the IAA concentration increasing The relative survival rates of C666-1 / HRP (+) group decreased gradually (15.58 ± 1.12%, (10.11 ± 1.04)%, (5.21 ± 1.04)%, respectively) The growth of pharyngeal carcinoma transplanted tumor was inhibited, the volume was significantly smaller than the empty virus group and the saline group (P <0.05), and the apoptosis of tumor cells was significantly increased (P <0.05). Conclusion The HRP / IAA enzyme prodrug system can kill NPC cell line C666-1 and has anti-nasopharyngeal carcinoma effect.