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目的探讨多点取材对食管癌ＤＮＡ倍体类型检测的影响及瘤体内ＤＮＡ倍体异质性的意义．方法８０例食管癌标本，采用了３点取材，用ＦＡＣＳ—４２０型流式细胞仪检测ＤＮＡ含量．对各取材点的ＤＮＡ倍体类型与患者的预后关系进行统计学分析．同时检出倍体异质性肿瘤．并对倍体异质性肿瘤患者的预后情况进行分析。结果Ａ、Ｂ、Ｃ各取材点的ＤＮＡ倍体类型与患者的预后均无相关性；倍体异质性肿瘤患者的预后劣于非异质性肿瘤患者。结论单点取材不能完全反映整个瘤体的倍体状态，其倍体类型不能反映患者的预后；ＤＮＡ倍体异质性与患者预后有关。
Objective To investigate the effect of multi-point sampling on the detection of DNA ploidy in esophageal cancer and the significance of DNA ploidy heterogeneity in tumors. Methods Eighty cases of esophageal cancer specimens were obtained using 3 points, and DNA content was detected by FACS-420 flow cytometer. Statistical analysis was performed on the relationship between the type of DNA ploidy and the prognosis of patients at each sampling point. At the same time detect ploidy heterogeneous tumors. The prognosis of patients with ploidy heterogeneous tumors was analyzed. Results There was no correlation between the DNA ploidy type and the prognosis of the patients in A, B and C sampling points; the prognosis of patients with ploidy heterogeneous tumors was worse than that of patients with non-heterogeneous tumors. Conclusion One-point sampling can not fully reflect the ploidy state of the whole tumor, and its ploidy type can not reflect the prognosis of patients; DNA ploidy heterogeneity is related to the prognosis of patients.