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目的建立人卵巢癌重症联合免疫缺陷小鼠(SCID鼠)移植瘤模型,为研究人腹水型卵巢癌的实验研究提供合适的动物模型。方法将2例卵巢癌患者手术切除标本移植于SCID鼠腹腔,原代移植瘤形成后进行鼠间传代,观察移植瘤的生长、转移及腹水形成情况,做腹水细胞学计数、涂片,检测荷瘤鼠血中肿瘤标记物CA125的浓度,肿瘤作病理学检查。结果成功建立3只原代人卵巢癌SCID鼠移植瘤模型,原代移植成功率为37.5%,自第四代后,移植瘤的传代成功率为100%,随着传代次数的增加,移植瘤的成瘤潜伏期及荷瘤鼠的生存期逐渐缩短,荷瘤鼠血中肿瘤标记物的浓度升高,病理组织学证实:移植瘤保持了原卵巢癌生物学特征。结论成功建立了人卵巢癌SCID鼠移植瘤模型,移植瘤保持了原卵巢癌生物学特征,是研究人卵巢癌较理想的动物模型。
OBJECTIVE: To establish a model of human ovarian cancer combined with severe immunodeficiency mice (SCID) xenograft model and to provide a suitable animal model for the study of human ascites-type ovarian cancer. Methods Two cases of ovarian cancer patients were transplanted into the peritoneal cavity of SCID mice. The primary xenografts were passaged after passage and the growth, metastasis and the formation of ascites were observed. The ascites cytology count, smear, test load Tumor blood tumor marker CA125 concentration, tumor for pathological examination. Results Three primary human ovarian cancer SCID mouse xenograft models were successfully established. The success rate of primary transplantation was 37.5%. After the fourth generation, the success rate of transplanted tumor was 100%. With the increase of passage times, The tumorigenic potential of tumor-bearing mice and the incubation period of tumor-bearing mice gradually shortened. The concentration of tumor markers in blood of tumor-bearing mice increased. Histopathology confirmed that the transplanted tumor maintained the biological characteristics of the original ovarian cancer. Conclusion The successful establishment of human ovarian cancer SCID mouse xenograft model, the xenograft tumor maintained the biological characteristics of ovarian cancer, ovarian cancer is more ideal animal model.