论文部分内容阅读
目的观察异氟醚对外周伤害性刺激大鼠模型脊髓ｃ－ｆｏｓ表达的影响，初步探讨异氟醚在脊髓的可能作用部位。方法 ＳＤ大鼠 ６０只随机分为 ３组。于大鼠左后掌心皮下注射 ５％福尔马林１５０ｕｌ建立外周伤害性刺激模型。Ａ组（伤害性刺激组，ｎ＝２５）。Ｂ组（异氟醚组，ｎ＝２５），在建立模型前１０ｍｉｎ开始吸入１．５ＭＡＣ异氟醚。Ａ、Ｂ组按刺激模型建立的时间（０ｈ， ０．５ｈ，１ｈ，２ｈ，３ｈ）随机分成５个亚组，０时点亚组用生理盐水代替福尔马林。Ｃ组（吗啡对照组，ｎ＝１０）建立模型前３０ｍｉｎ腹腔注射吗啡 １０ｍｇ·ｋｇ－１，５ｍｉｎ后其中 ５只加注纳络酮 ２ｍｇ·ｋｇ－１，均存活 ２ｈ。切片用免疫组织化学法显示Ｆｏｓ阳性神经元。结果Ａ组Ｆｏｓ阳性神经元分布以Ⅰ～Ⅱ层为主，吗啡预处理能抑制上述表达，而纳络酮能使上述抑制作用翻转； １． ７３％异氟醚主要抑制 Ⅰ～Ⅱ层各时点 Ｆｏｓ蛋白在大鼠脊髓背角灰质的表达。结论脊髓灰质后角Ⅰ～Ⅱ层可能是异氟醚抗外周伤害性刺激作用的关键部位。
Objective To investigate the effect of isoflurane on the expression of c-fos in spinal cord of peripheral nociceptive rats, and to explore the possible role of isoflurane in the spinal cord. Methods Sixty SD rats were randomly divided into three groups. A rat model of peripheral noxious stimulation was established by subcutaneous injection of formalin 150ul 5% into the left palm of rats. Group A (noxious stimulation group, n = 25). In group B (isoflurane group, n = 25), inhalation of 1.5MAC isoflurane 10 min before model establishment. Group A and group B were randomly divided into 5 subgroups according to the time of establishment of the stimulation model (0h, 0.5h, 1h, 2h, 3h). At 0 o’clock subgroup, normal saline was used instead of formalin. In group C (morphine control group, n = 10), 5 rats were injected with 2 mg · kg-1 naloxone after morphine 10 mg · kg-1 and injection for 30 min before the model was established. Fos-positive neurons were visualized by immunohistochemistry. Results The distribution of Fos positive neurons in group A mainly consisted of layer Ⅰ ~ Ⅱ. Morphine pretreatment inhibited the above expression, while naloxone reversed the inhibitory effect. 73% isoflurane mainly inhibits the expression of Fos protein in the dorsal horn of spinal dorsal horn of rats in each layer Ⅰ ~ Ⅱ. Conclusions The Ⅰ ~ Ⅱ layers of the posterior horn of the gray matter of the spinal cord may be the key part of isoflurane for the peripheral noxious stimulation.