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目的:观察重组人粒细胞刺激因子(rh G-CSF)对肝纤维化大鼠的修复作用。方法:选取清洁级Wistar大鼠30只分为3组,即正常组、肝纤维化模型组(等量生理盐水)、实验组(rh G-CSF 200μg/kg·d),每组各10只,除正常组外均采用四氯化碳(CCl4)综合法建立肝纤维化模型,造模成功后,皮下注射相应药物1周,处死大鼠后,留取血清、肝组织,检测丙氨酸氨基转移酶(ALT)、白蛋白(ALB)、总胆红素(TBIL)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、肌酸激酶(CK)、透明质酸(HA)、层粘连蛋白(LN)、羟脯氨酸(Hyp)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)等肝功能及肝纤维化指标的变化,采用HE染色及Masson染色观察各组大鼠肝组织病理变化情况。结果:1与对照组相比,模型组、实验组大鼠血清ALT、AST、TBIL均上升(P<0.05),ALB均降低(P<0.05);与模型组对比,实验组ALT、AST、TBIL降低,ALB升高(P<0.05);2与对照组相比,模型组、实验组HA、LN、PCⅢ、Ⅳ-C上升(P<0.05);与模型组对比,实验组HA、LN、PCⅢ、Ⅳ-C均降低,对比差异有统计学意义(P<0.05);3与对照组相比,模型组、实验组ALP、CK上升(P<0.05);与模型组对比,实验组ALP、CK降低,对比差异有统计学意义(P<0.05);4与对照组相比,模型组、实验组Hy P上升(P<0.05);与模型组对比,实验组Hy P降低,对比差异有统计学意义(P<0.05)。结论:rh G-CSF可促进肝纤维化大鼠肝功能修复,减轻肝纤维化程度。
Objective: To observe the effect of recombinant human granulocyte-stimulating factor (rhG-CSF) on the repair of hepatic fibrosis in rats. Methods: Thirty healthy Wistar rats were divided into 3 groups: normal group, hepatic fibrosis model group (equal volume of normal saline), and experimental group (rhG-CSF 200μg / kg · d) , Except for normal group, liver fibrosis model was established by carbon tetrachloride (CCl4) synthesis method. After the model was successfully established, the corresponding drugs were subcutaneously injected for 1 week. After the rats were sacrificed, serum and liver tissues were collected for detection of alanine (ALT), albumin (ALB), total bilirubin (TBIL), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), hyaluronic acid (HA), laminin (LN), hydroxyproline (Hyp), type III procollagen (PCⅢ) and type Ⅳ collagen (Ⅳ-C) Masson staining was used to observe the pathological changes of liver tissue in each group. Compared with the control group, ALT, AST and TBIL in model group and experimental group increased (P <0.05), ALB decreased (P <0.05) (P <0.05). Compared with the control group, the levels of HA, LN, PCⅢ, Ⅳ-C in the model group and the experimental group increased (P <0.05). Compared with the model group, (P <0.05). Compared with the control group, ALP and CK in the model group and experimental group increased (P <0.05). Compared with the model group, the experimental group (P <0.05) .4Compared with the control group, the Hy P of the model group and the experimental group increased (P <0.05). Compared with the model group, the Hy P of the experimental group decreased The difference was statistically significant (P <0.05). Conclusion: rhG-CSF can promote liver function in rats with liver fibrosis and reduce the degree of liver fibrosis.