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目的检测β-连接蛋白(β-catenin)、周期素D1(cyclin D1)和雌激素受体α(ERα)在乳腺癌中的表达,探讨其在乳癌发病机制中的作用。方法随机选取42例乳腺增生,58例乳腺癌病人的乳腺组织以及乳腺癌灶周围正常乳腺组织,分别应用免疫组化SP法检测β-catenin,cyclin D1的表达,应用Western-blot法测ERα的表达。结果乳腺癌组织β-catenin呈胞质和/或胞核异位表达,其表达率为65.5%,显著高于乳腺增生和正常乳腺组织(42.9%、35.7%,P<0.05)。乳腺癌cyclin D1和ERα阳性率分别为74.1%、70.7%,均显著高于乳腺增生和正常乳腺组织(45.2%、36.5%;33.3%、29.5%;P均<0.01)。乳腺癌中β-catenin与cyclin D1和ERα表达分别呈明显正相关(r=0.32、0.57,P<0.05、P<0.01)。结论乳腺癌中ERα、cyclin D1和β-catenin均高表达,且呈正相关,提示三者在乳腺癌发病中具有协同作用。
Objective To detect the expression of β-catenin, cyclin D1 and ERα in breast cancer, and to explore its role in the pathogenesis of breast cancer. Methods 42 cases of breast hyperplasia, 58 cases of breast cancer patients and normal breast tissue around the breast cancer tissue were collected. The expression of β-catenin and cyclin D1 were detected by immunohistochemical SP method. The expression of ERα expression. Results The expression of β-catenin in cytoplasm and / or nucleus of breast cancer tissues was significantly higher than that in normal breast tissues (42.9%, 35.7%, P <0.05). The positive rates of cyclin D1 and ERα in breast cancer were 74.1% and 70.7%, respectively, which were significantly higher than those in breast hyperplasia and normal breast tissues (45.2%, 36.5%; 33.3%, 29.5%, P <0.01). Β-catenin in breast cancer was positively correlated with cyclin D1 and ERα (r = 0.32,0.57, P <0.05, P <0.01). Conclusion The expressions of ERα, cyclin D1 and β-catenin in breast cancer are highly correlated, which indicates that they have synergistic effects in the pathogenesis of breast cancer.