论文部分内容阅读
AIM:To investigate the effects of allicin on both telomeraseactivity and apoptosis in gastric cancer SGC-7901 cells.METHODS:The gastric cancer SGC-7901 adenocarcinomacells were treated with allicin and the cell cycle,inhibitoryrate,apoptosis,telomerase activity and morphologic changeswere studied by MTT assay,flow cytometry(FCM),TRAP-PCR-ELISA assay,light microscope,electron microscoperespectively.Results were compared with that of AZT(3’-Azido-3’-deoxythymidine).RESULTS:SGC-7901 cells were suppressed after exposureto allicin of 0.016 mg/ml,0.05 mg/ml,and 0.1 mg/ml for48 h.Compared with the control,the difference wassignificant(P<0.05).Allicin could induce apoptosis of thecells in a dose-dependent and non-linear manner andincrease the proportion of cells in the G_2/M phase.Comparedwith the control,the difference was significant in terms ofthe percentage of cells in the G_2/M phase(P<0.05).Allicincould inhibit telomerase activity in a time-dependent anddose-dependent pattern.After exposure to allicin at 0.016mg/ml for 24 hours,SGC-7901 cells showed typicalmorphologic change.CONCLUSION:Allicin can inhibit telomerase activity andinduce apoptosis of gastric cancer SGC-7901 cells.Allicinmay be more effective than AZT.
AIM: To investigate the effects of allicin on both telomerase activity and apoptosis in gastric cancer SGC-7901 cells. METHODS: The gastric cancer SGC-7901 adenocarcinoma cells were treated with allicin and the cell cycle, inhibitory rate, apoptosis, telomerase activity and morphologic changes were studied by The results of the MTT assay, flow cytometry (FCM), TRAP-PCR-ELISA assay, light microscope, electron microscope were positive. Results were compared with that of AZT (3’-Azido-3’-deoxythymidine) .RESULTS: SGC-7901 cells were suppressed after Exposure to allicin of 0.016 mg / ml, 0.05 mg / ml, and 0.1 mg / ml for 48 h. Compared with the control, the difference was significant (P <0.05) .Allicin could induce apoptosis of the cells in a dose-dependent and non-linear manner and increase the proportion of cells in the G_2 / M phase. Compared with the control, the difference was significant in terms of the percentage of cells in the G_2 / M phase (P <0.05). Allicincould inhibit telomerase activity in a time-dependent and dose- dependent pattern.After expos ure to allicin at 0.016 mg / ml for 24 hours, SGC-7901 cells showed typical morphologic change. CONCLUSION: Allicin can inhibit telomerase activity and apoptosis of gastric cancer SGC-7901 cells. Allicin be more effective than AZT.