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通过化学还原法制备石墨烯(GR)、石墨烯/金(GR/Au)复合纳米材料,利用TEM,EDS对所合成的纳米复合材料的结构和形貌进行表征。GR/Au表现出对酪氨酸酶(Tyr)强烈的吸附性,构建GR/Au-Tyr-CS/GCE修饰电极。该修饰电极不仅可实现Tyr与电极间的直接电子转移,而且对双酚A表现出良好的催化性能。在工作电位0~1V,0.1 mol/L磷酸盐缓冲盐溶液(PBS,pH 7.0)中,该酶传感器对双酚A响应灵敏度高,检测范围宽,其催化的线性范围为0~2.5×10-6 mol/L(相关系数0.99653),检出限为1.88×10-8 mol/L(S/N=3),表现出良好的稳定性和重现性。
The graphene (GR) and graphene / gold (GR / Au) nanocomposites were prepared by chemical reduction method. The structure and morphology of the synthesized nanocomposites were characterized by TEM and EDS. GR / Au showed strong adsorption to tyrosinase (Tyr) and the GR / Au-Tyr-CS / GCE modified electrode was constructed. The modified electrode can not only realize direct electron transfer between Tyr and electrode, but also exhibit good catalytic performance for bisphenol A. The sensitivity of the enzyme sensor to bisphenol A was high in the range of 0 ~ 1 V and 0.1 mol / L phosphate buffered saline (PBS, pH 7.0). The linear range of the enzyme was 0 ~ 2.5 × 10 -6 mol / L (correlation coefficient of 0.99653). The detection limit was 1.88 × 10-8 mol / L (S / N = 3), showing good stability and reproducibility.