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目的探讨细胞因子信号抑制因子(SOCS)在支气管哮喘(简称哮喘)患儿TH细胞亚群(TH1/TH2)功能失衡中的作用,以及转录因子T-bet和GATA3与TH1／TH2反应的关系。方法观察20例哮喘患儿及相同数量同龄对照,采用流式细胞术(FCM)检测哮喘患者外周血CD4+T淋巴细胞浆中自细胞介素4(IL-4)和γ干扰素(INF-γ)的表达,逆转录-聚合酶链反应(RT-PCR)和荧光定量聚合酶链反应(real time PER)测定淋巴细胞SOCS3、SOCX5、T-bet、GATA3 mRNA表达水平。结果急性发作期哮喘息儿TH1细胞比例显著下降,TH2细胞显著增高(P<0．01);哮喘患儿淋巴细胞SOCS3、GATA3 mRNA表达水平显著高于正常同龄对照(P<0．01),SOCX5、T-bet mRNA表达显著下降(P<0．01)。结论SOCS3、SOCS5、T-bet和GATA3表达异常与哮喘患儿TH1／TH2失衡有关,其中SOC33和SOCS5表达异常可能是重要的因素之一。
Objective To investigate the role of cytokine signaling inhibitor (SOCS) in imbalance of TH cell subsets (TH1 / TH2) in children with bronchial asthma (asthma) and the relationship between T-bet, GATA3 and TH1 / TH2 responses. Methods 20 cases of asthmatic children and the same number of the same age control were observed by flow cytometry (FCM) in peripheral blood of asthmatic patients with CD4 + T lymphocytes in the plasma levels of interleukin-4 and interferon-gamma (INF- γ, and the expression of SOCS3, SOCX5, T-bet and GATA3 in lymphocytes were determined by reverse transcription-polymerase chain reaction (RT-PCR) and real time PER. Results The percentage of TH1 cells in asthmatic children decreased significantly during acute exacerbation and TH2 cells were significantly increased (P <0.01). The expression of SOCS3 and GATA3 mRNA in lymphocytes of asthmatic children was significantly higher than that of normal controls (P <0.01) SOCX5, T-bet mRNA expression was significantly decreased (P <0.01). Conclusion The abnormal expressions of SOCS3, SOCS5, T-bet and GATA3 are related to imbalance of TH1 / TH2 in children with asthma. The abnormal expression of SOC33 and SOCS5 may be one of the important factors.