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目的:探讨纳米雄黄对人皮肤鳞癌A431细胞株增殖抑制及诱导凋亡作用及作用机制。方法:应用MTT比色法、流式细胞仪观察纳米雄黄体对人皮肤鳞状细胞癌A431细胞的增殖及凋亡情况、RT-PCR检测纳米雄黄对生存素(Survivin)mRNA,Caspase-3 mRNA表达的影响。结果:MTT实验及流式细胞仪提示纳米雄黄具有诱导皮肤鳞癌A431细胞凋亡和抑制细胞增殖作用,且随着纳米雄黄剂量的增加作用增强,与顺铂联合应用有协同作用;对照组,5%,10%,20%纳米雄黄组,DDP组,DDP+10%纳米雄黄组的Survivin相对表达量依次为(68.74±1.96)%,(63.93±3.57)%,(57.12±3.93)%,(47.64±6.44)%,(41.44±4.83)%及(32.18±4.10)%;Casepase-3相对表达量依次为(26.26±2.07)%,(32.660±4.42)%,(35.29±5.26)%,(38.52±8.37)%,(45.69±4.19)%及(52.98±6.52)%;纳米雄黄剂量增加可促进Caspase-3的表达,降低Survivin的表达。结论:纳米雄黄可通过诱导肿瘤细胞凋亡和抑制其增殖发挥抗肿瘤作用;其作用机制可能与上调Caspase-3的表达和下调Survivin的表达有关。
Objective: To investigate the inhibitory effect of nano-realgar on proliferation and apoptosis of human skin squamous cell carcinoma A431 cell line and its mechanism. Methods: The proliferation and apoptosis of human skin squamous cell carcinoma A431 cells were observed by MTT assay and flow cytometry. The effects of nano-realgar on the expression of Survivin mRNA, Caspase-3 mRNA The impact of expression. Results: MTT assay and flow cytometry suggested that nano realgar could induce the apoptosis of A431 cells and inhibit the cell proliferation. With the increase of the dose of nano-realgar, the effect of nano-realgar increased and the synergistic effect with cisplatin. In the control group, The relative expression levels of Survivin in 5%, 10%, 20% nano-realgar group, DDP group and DDP + 10% nano-real yellow group were 68.74 ± 1.96%, 63.93 ± 3.57%, 57.12 ± 3.93% (47.64 ± 6.44)%, (41.44 ± 4.83)% and (32.18 ± 4.10)%, respectively. The relative expression levels of Casepase-3 were (26.26 ± 2.07)%, (32.660 ± 4.42)% and (35.29 ± 5.26)%, (38.52 ± 8.37)%, (45.69 ± 4.19)% and (52.98 ± 6.52)%, respectively. The increase of the dose of nano-realgar could promote the expression of Caspase-3 and decrease the expression of Survivin. CONCLUSION: Nano realgar can exert anti-tumor effect by inducing tumor cell apoptosis and inhibiting its proliferation. Its mechanism may be related to the up-regulation of Caspase-3 expression and down-regulation of Survivin expression.