论文部分内容阅读
目的观察基质金属蛋白酶1(matrixmetalloproteinase1,MMP1)在川崎病患儿外周血不同时期的表达水平,探讨其在冠状动脉(简称冠脉)损伤中的作用。方法40例川崎病患儿(无冠脉损伤组23例,冠脉损伤组17例),按病程分为急性期、亚急性期和恢复期;另分别以10例败血症患儿、10例健康儿童为发热对照组和正常对照组。应用酶联免疫吸附法检测血清MMP1蛋白水平、逆转录聚合酶链反应(RTPCR)检测外周血白细胞MMP1mRNA表达水平。结果急性期有、无冠脉损伤组血清MMP1和白细胞表达MMP1mRNA水平均明显高于发热对照组和正常对照组(均P<0.01),且冠脉损伤组MMP1蛋白和mRNA水平较无冠脉损伤组升高更为显著(P<0.05);到亚急性期、恢复期时MMP1蛋白和mRNA水平依次明显降低(各期间比较,均P<0.01)。川崎病组急性期血清MMP1蛋白水平与外周血白细胞计数呈显著正相关(r=0.750,P<0.01)。结论MMP1在川崎病急性期,尤其在冠脉损伤时表达明显升高;MMP1的过度表达可能与川崎病冠脉损伤的形成有关。
Objective To investigate the expression of matrix metalloproteinase 1 (MMP-1) in peripheral blood of children with Kawasaki disease at different stages and to explore the role of matrix metalloproteinase 1 in coronary artery injury. Methods 40 children with Kawasaki disease (23 without coronary artery injury and 17 with coronary artery injury) were divided into acute phase, subacute phase and convalescent phase according to the course of disease. Another 10 children with Kawasaki disease and 10 healthy children Children as fever control group and normal control group. Serum MMP1 protein levels were detected by enzyme-linked immunosorbent assay, and MMP1 mRNA expression in peripheral leukocytes was detected by reverse transcriptase-polymerase chain reaction (RTPCR). Results The levels of serum MMP1 and leukocyte-derived MMP1 mRNA in acute stage and without coronary artery injury were significantly higher than those in fever control group and normal control group (all P <0.01). The levels of MMP1 protein and mRNA in coronary artery injury group were significantly lower than those in non-coronary group (P <0.05). In the subacute and convalescent periods, MMP1 protein and mRNA levels decreased significantly (P <0.01 for all periods). Kawasaki disease acute exacerbation of serum MMP1 protein levels and peripheral leukocyte count was significantly correlated (r = 0.750, P <0.01). Conclusion The expression of MMP1 is significantly increased in acute Kawasaki disease, especially in coronary artery injury. The overexpression of MMP1 may be related to the formation of coronary artery injury in Kawasaki disease.