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北斗是我场从日本引进的最新苹果优良品种。为了更快地提供无病毒北斗果苗,从 1987年春开始,进行了离体茎尖培养,其主要技术如下。 一外殖体选择、消毒及茎尖接种 把带有营养芽的嫩枝去叶后,剪成2一3cm长茎段,每段具有一个营养芽,剥掉其韧皮部,然后用70%酒精浸洗1分钟左右,再倒
Compass is my field from Japan to introduce the latest Apple fine varieties. In order to provide virus-free Beodo seedlings more quickly, from the spring of 1987, the in vitro shoot tip culture was carried out. The main techniques are as follows. A selection of exosomes, disinfection and shoot inoculation The shoots with vegetative shoots were removed and cut into 2 to 3 cm long stem segments, each with a vegetative shoot, stripped of its phloem, and then diluted with 70% alcohol Wash about 1 minute, then pour