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目的 :探讨慢性乙型肝炎以及肝硬化患者肠道微生物分布状况。方法 :于2015年3月到2016年3月间,在我疾控中心选择40例慢性乙型肝炎患者、40例肝硬化患者及40例健康体检者作为研究对象,取所有人员的粪便,采取实时荧光定量PCR法测定并对比三组人员粪便中9种常见肠道菌群含量状况。结果:慢性乙型肝炎组和肝硬化组患者肠球菌、肠杆菌科细菌、乳酸杆菌、双歧杆菌、梭菌属、白色念珠菌、拟杆菌属、普雷沃氏菌属、瘤胃球菌属计数水平与健康体检组相比均存在显著差异(P<0.05)。且肝硬化组患者乳酸杆菌、瘤胃球菌属计数水平与慢性乙型肝炎组患者相比明显较高,P<0.05。结论 :慢性乙型肝炎以及肝硬化患者肠道微生物分布存在不同程度的菌落失调状况。
Objective: To investigate the distribution of intestinal microflora in patients with chronic hepatitis B and cirrhosis. Methods: From March 2015 to March 2016, 40 chronic hepatitis B patients, 40 cirrhotic patients and 40 healthy subjects were selected as research subjects in our CDC. Real-time fluorescence quantitative PCR method was used to measure and compare the content of 9 kinds of common intestinal flora in feces of three groups. Results: Enterococcus, Enterobacteriaceae, Lactobacillus, Bifidobacterium, Clostridium, Candida albicans, Bacteroides, Prevotella, Rumenococcus count in patients with chronic hepatitis B and cirrhosis There were significant differences between the two groups (P <0.05). The counts of Lactobacillus and Ruminococcus in patients with cirrhosis were significantly higher than those in patients with chronic hepatitis B (P <0.05). Conclusion: The distribution of intestinal microflora in patients with chronic hepatitis B and cirrhosis has different degrees of colony imbalance.