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目的探讨兔颈动脉粥样硬化斑块中环氧化物酶-2(COX-2)、诱导型前列腺素合成酶-1(mPGES-1)的表达机制及塞来昔布对其表达的影响。方法采用32只雄性新西兰大白兔,其中正常对照组8只(A组),另24只将特制的硅橡胶圈置于兔右侧颈动脉,术后给予1%高胆固醇喂养14d,建立兔颈动脉粥样硬化性狭窄动物模型,将模型兔随机分为颈动脉狭窄无干预组(B组)、小剂量塞来昔布治疗组(15 mg/kg,C组),大剂量塞来昔布治疗组(35 mg/kg,D组),每组各8只。治疗4周后取双侧颈动脉并分为平等2段,分别行半定量逆转录聚合酶链反应(RT-PCR)及Western blot法测定颈动脉斑块干预后COX-2及mPGES-1的表达。结果与A组比较,B、C、D组颈动脉粥样硬化模型兔的斑块中COX-2及mPGES-1表达增高,差异具有显著性(P<0.05);与B组比较,C、D组颈动脉斑块的COX-2及mPGES-1表达显著下调,差异有显著性(P<0.05)。结论在颈动脉粥样硬化斑块的病理过程中炎症反应可能起着主导作用,塞来昔布干预可抑制COX-2及mPGES-1的表达,减缓颈动脉粥样硬化的进展。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) and inducible prostaglandin synthase-1 (mPGES-1) in rabbit carotid atherosclerotic plaque and the effect of celecoxib on it. Methods Thirty-two male New Zealand white rabbits were randomly divided into two groups: normal control group (A group), the other 24 rabbits were placed in the right rabbit carotid artery, and 1% Atherosclerotic stenosis animal model, the model rabbits were randomly divided into no carotid artery stenosis group (B group), low-dose celecoxib treatment group (15 mg / kg, C group), high-dose celecoxib Treatment group (35 mg / kg, D group), each group of eight. After 4 weeks of treatment, bilateral carotid arteries were obtained and divided into 2 equal sections. The expression of COX-2 and mPGES-1 in plaque of carotid arteries were detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) expression. Results Compared with group A, the expression of COX-2 and mPGES-1 in carotid atherosclerosis model rabbits in groups B, C and D increased significantly (P <0.05). Compared with group B, C, The expression of COX-2 and mPGES-1 in carotid artery plaque in group D were significantly decreased (P <0.05). Conclusion Inflammatory reaction may play a leading role in the pathological process of carotid atherosclerotic plaque. Celecoxib can inhibit the expression of COX-2 and mPGES-1, and slow down the progression of carotid atherosclerosis.