Objective:To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County,using ELISA method.Methods:Based on the prevalence and characteristics method,400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases,Southern Iran,during 2010–2011,designed at testing by ELISA.Ig M and Ig G antibodies against Toxoplasma gondii were tested using ELISA kits(Dia-Pro)on serums.The data were analysed by SPSS 19 software.Results:Review of 400 cases,54 of them were Ig G positive for parasites(13.5%)and 346of those with negative Ig G(86.5%).In Ig M examination,1.75%of them have been positive Ig M(7 cases)and 98.25%of them were Ig M negative(393 cases).By comparing the different group ages,40–50 year age group had the highest prevalence of Ig G positive(17.9%)and the age group of 30–40 years had the highest incidence of Ig M negative(2.5%).Conclusions:Due to the serological infection rate of toxoplasmosis obtained from this study,toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations. Objective: To identify the anti-Toxoplasma antibodies from blood donors who referred to blood transfusion bases of Jahrom County, using ELISA method. Methods: Based on the prevalence and characteristics method, 400 serum samples were collected from blood donors referred to Jahrom blood transfusion bases , Southern Iran, during 2010-2011, designed at testing by ELISA. Ig M and Ig G antibodies against Toxoplasma gondii were tested using ELISA kits (Dia-Pro) on serums. The data were analyzed by SPSS 19 software. Results: Review of Of the 400 cases, 54 of them were Ig G positive for parasites (13.5%) and 346 of those with negative Ig G (86.5%). In Ig M examination, 1.75% of them have been positive Ig M (7 cases) and 98.25% of they were Ig M negative (393 cases) .By comparing the different group ages, 40-50 year age group had the highest prevalence of Ig G positive (17.9%) and the age group of 30-40 years had the highest incidence of Ig M negative (2.5%). Conclusions: Due to the serological infection rate of toxoplasmosis ob tained from this study, toxoplasmosis should be considered as a significant transfusion risk factor in Jahrom and also in any region with similar situations.