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水稻糙米率是加工品质中的一个重要组成部分。为了探索糙米率的遗传基础,本研究以V20B和CPSLO17作为亲本,构建了150个重组自交系(recombinant inbred lines,RIL)群体。利用SLAF标签构建的精度更高、平均遗传距离为0.292 c M的高密度遗传图谱,结合亲本和群体糙米率表型数据,对三亚和贵阳两个环境下控制水稻糙米率的数量性状位点(quantitative trait loci,QTL)进行遗传分析。结果显示:三亚和贵阳共检测到两个QTL。其中,三亚检测到1个QTL位点q BR1,位于第1染色体Marker600937~Marker685097区间上,两个标记间遗传距离为0.471 c M,贡献率为9.7470%;贵阳检测到1个QTL位点q BR4位于第4染色体Marker503771~Marker431234区间上,两个标记间遗传距离为0.469 c M,贡献率为9.7634%。两个检测到的QTL,在两个环境中未重复检测到,且增效位点均来自于亲本V20B。本研究对进一步发掘和利用水稻糙米率QTL具有重要意义,同时为利用分子标记辅助选择提高水稻糙米率提供参考。
Brown rice rate is an important part of processing quality. In order to explore the genetic basis of brown rice rate, we constructed 150 populations of recombinant inbred lines (RILs) using V20B and CPSLO17 as their parents. Based on the high-density genetic map constructed by SLAF, the average genetic distance was 0.292 cM. Based on the phenotypic data of parents and population of brown rice, the quantitative trait loci quantitative trait loci (QTL) for genetic analysis. The results showed that two QTLs were detected in Sanya and Guiyang. Among them, one QTL locus q BR1 was detected in Sanya, located on the interval of Marker600937 ~ Marker685097 on chromosome 1, with a genetic distance of 0.471 cM between the two markers and a contribution rate of 9.7470%. A QTL locus q BR4 Located on the chromosome 4 Marker503771 ~ Marker431234 interval, the genetic distance between the two markers was 0.469 c M, the contribution rate of 9.7634%. The two detected QTLs were not detected repeatedly in both environments, and all of the synergistic sites were from the parent V20B. This study is of great significance for the further exploration and utilization of QTL for brown rice rate in rice, and provides a reference for improving the brown rice rate using molecular marker-assisted selection.