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目的:利用超高效液相-质谱法建立同时测定降脂颗粒中6种活性成分含量的方法。方法:色谱柱为Acquity UPLC BEH C18柱(2.1mm×50mm,1.7μm),流动相为甲醇-0.1%甲酸水梯度洗脱,选择离子监测。结果:在线性范围内相关系数良好,均大于0.9992,日内、日间精密度良好,RSD值均小于3%,加样回收率在97.58%-103.12%,符合要求。结论:本方法快速灵敏,测定结果准确可靠,可用于降脂颗粒的质量控制。
OBJECTIVE: To establish a method for simultaneous determination of 6 active ingredients in Jiangzhi granules by ultra performance liquid chromatography-mass spectrometry. Methods: The column was Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm). The mobile phase consisted of a gradient of methanol-0.1% formic acid and selected for ion monitoring. Results: The correlation coefficients were all within 0.9992 in the linear range. The precision was good in the day and in the day. The RSD values were less than 3% and the recoveries were 97.58% -103.12%, which met the requirements. Conclusion: The method is rapid and sensitive, the determination results are accurate and reliable, and can be used for quality control of lipid-lowering granules.